Thymidylate synthase (FAD)

This enzyme belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. The systematic name of this enzyme class is 5,10-methylenetetrahydrofolate,FADH2:dUMP C-methyltransferase. Other names in common use include Thy1, and ThyX. This enzyme participates in pyrimidine metabolism and one carbon pool by folate.

Structural studies

As of late 2007, 3 structures have been solved for this class of enzymes, with PDB accession codes 2AF6, 2CFA, and 2GQ2.

References

Myllykallio H, Lipowski G, Leduc D, Filee J, Forterre P, Liebl U (2002). "An alternative flavin-dependent mechanism for thymidylate synthesis". Science. 297 (5578): 105–7. doi:10.1126/science.1072113. PMID 12029065.

Transferase: one carbon transferases (EC 2.1)

2.1.1: Methyl-

N-	Histamine N-methyltransferase - Phenylethanolamine N-methyltransferase - Amine N-methyltransferase - Phosphatidylethanolamine N-methyltransferase

O-	5-hydroxyindole-O-methyltransferase/Acetylserotonin O-methyltransferase - Catechol-O-methyl transferase

Homocysteine	Betaine-homocysteine methyltransferase - Homocysteine methyltransferase - Methionine synthase

Other	Phosphatidyl ethanolamine methyltransferase - DNMT3B - Histone methyltransferase - Thymidylate synthase - DNA methyltransferase - Thiopurine methyltransferase

2.1.2: Hydroxymethyl-,
Formyl- and Related

Hydroxymethyltransferase	Serine hydroxymethyltransferase - 3-methyl-2-oxobutanoate hydroxymethyltransferase

Formyltransferase	Phosphoribosylglycinamide formyltransferase - Inosine monophosphate synthase

Other	Glutamate formimidoyltransferase - Aminomethyltransferase

2.1.3: Carboxy- and Carbamoyl

Carboxy	methylmalonyl-CoA carboxytransferase

Carbamoyl	Aspartate carbamoyltransferase - Ornithine carbamoyltransferase - Oxamate carbamoyltransferase - Putrescine carbamoyltransferase - 3-hydroxymethylcephem carbamoyltransferase - Lysine carbamoyltransferase - N-acetylornithine carbamoyltransferase

2.1.4: Amidino

Arginine:glycine amidinotransferase

B enzm: 1.1/2/3/4/5/6/7/8/10/11/13/14/15-18, 2.1/2/3/4/5/6/7/8, 2.7.10, 2.7.11-12, 3.1/2/3/4/5/6/7, 3.1.3.48, 3.4.21/22/23/24, 4.1/2/3/4/5/6, 5.1/2/3/4/99, 6.1-3/4/5-6

Metabolism: amino acid metabolism · nucleotide enzymes

Purine metabolism

Anabolism	R5P->IMP: Ribose-phosphate diphosphokinase · Amidophosphoribosyltransferase · Phosphoribosylglycinamide formyltransferase · AIR synthetase (FGAM cyclase) · Phosphoribosylaminoimidazole carboxylase · Phosphoribosylaminoimidazolesuccinocarboxamide synthase · IMP synthase IMP->AMP: Adenylosuccinate synthase · Adenylosuccinate lyase · reverse (AMP deaminase) IMP->GMP: IMP dehydrogenase · GMP synthase · reverse (GMP reductase)

Nucleotide salvage	Hypoxanthine-guanine phosphoribosyltransferase · Adenine phosphoribosyltransferase

Catabolism	Adenosine deaminase · Purine nucleoside phosphorylase · Guanine deaminase · Xanthine oxidase · Urate oxidase

Pyrimidine metabolism

Anabolism	CAD (Carbamoyl phosphate synthase II, Aspartate carbamoyltransferase, Dihydroorotase) Dihydroorotate dehydrogenase · Orotidine 5'-phosphate decarboxylase/Uridine monophosphate synthetase CTP synthase

Catabolism	Dihydropyrimidine dehydrogenase · Dihydropyrimidinase/DPYS · Beta-ureidopropionase/UPB1

Deoxyribonucleotides

Ribonucleotide reductase · Nucleoside-diphosphate kinase · DCMP deaminase · Thymidylate synthase · Dihydrofolate reductase

M: MET

mt, k, c/g/r/p/y/i, f/h/s/l/o/e, a/u, n, m

k, cgrp/y/i, f/h/s/l/o/e, au, n, m, epon

m(A16/C10),i(k, c/g/r/p/y/i, f/h/s/o/e, a/u, n, m)

Thymidylate synthase (FAD)

Structural studies

See also

References